@article {4541,
	title = {Human herpesvirus 8 transfusion transmission in Ghana, an endemic region of West Africa.},
	journal = {Transfusion// Transfusion},
	volume = {52},
	year = {2012},
	month = {2012//},
	pages = {2294 - 9},
	address = {United States},
	abstract = {BACKGROUND: Human herpesvirus 8 (HHV-8) seroprevalence ranges between less than 5\% in Europe and North America and 50\% to 70\% in sub-Saharan Africa. Evidence of HHV-8 transfusion transmission is only indirect. We conducted a serologic (anti-HHV-8) and molecular (HHV-8 DNA) study of samples from paired donor-immunocompetent recipients transfused with whole blood., STUDY DESIGN AND METHODS: Samples from 252 donor-recipient pairs were tested. Immunoglobulin G to HHV-8 was detected with enzyme immunoassays and confirmed with an in-house immunofluorescence assay. The cellular fraction from seroreactive donors and their recipients was tested for HHV-8 DNA., RESULTS: Anti-HHV-8 was positive (reactive in two or more assays) in 28 (11\%) patients and 16 (6\%) donors. Of 12 seronegative recipients (at risk of transmission) receiving seropositive blood, one very likely transmission was identified (8.3\% confidence interval, 0\%-23\%). The donor blood contained HHV-8 DNA and his and four other donors{\textquoteright} sequences clustered separately from recorded genotypes with a 97\% bootstrap constituting a distinct genotype., CONCLUSIONS: HHV-8 is transmitted in Ghana but does not carry clinical consequences since most patients are immunocompetent. The clinical risk will increase with the availability of immunosuppressive drugs in sub-Saharan Africa. We propose that a new genotype (HHV-8-G for Ghana) be added to the current nomenclature.Copyright {\textcopyright} 2012 American Association of Blood Banks.},
	keywords = {*Blood Transfusion/ae [Adverse Effects], *Blood Transfusion/sn [Statistics \& Numerical Data], *Endemic Diseases/sn [Statistics \& Numerical Data], *Herpesviridae Infections, *Herpesvirus 8, Human/ge [Genetics], Antibodies, Viral/bl [Blood], Blood Donors/sn [Statistics \& Numerical Data], Genotype, Ghana/ep [Epidemiology], Guinea/ep [Epidemiology], Herpesviridae Infections/bl [Blood], Herpesviridae Infections/ep [Epidemiology], Herpesviridae Infections/tm [Transmission], Herpesvirus 8, Human/cl [Classification], Herpesvirus 8, Human/im [Immunology], Humans, Phylogeny, Risk Factors, Seroepidemiologic Studies},
	isbn = {1537-2995},
	author = {Gobbini, Francesca and Owusu-Ofori, Shirley and Marcelin, Anne-Genevieve and Candotti, Daniel and Allain, Jean-Pierre}
}
