@article {4896,
	title = {Long-term persistent lymphopenia in hematopoietic stem cell donors after donation for donor lymphocyte infusion},
	volume = {32},
	year = {2004},
	month = {Nov},
	abstract = {Objectives: To analyze the consequences of lymphocyte donations on lymphopoiesis in donors having previously undergone hematopoietic stem cell collection for allogeneic stem cell transplantation.

Methods: Repeated analysis of leukocyte subsets in the peripheral blood of 76 hematopoietic stem cell donors undergoing lymphocyte donation(s) for DLI.

Results: Grade I/II lymphopenia was present in 22 donors (29\%) just before first apheresis for lymphocyte collection, demonstrating that former stem cell donation induced prolonged lymphopenia in a subset of donors. The monocytic lineage was not affected. Older age and history of PBSC harvest constituted 2 independent factors of lymphopenia, but had no influence on monocytopenia. The first apheresis induced lymphopenia in 36 donors (47\%) and monocytopenia in 23 donors (39\%). Lymphopenia before first apheresis and prior history of PBSC harvest were independent factors of apheresis-induced lymphopenia while those factors had no influence on monocytopenia. A time-dependent decrease in lymphocyte counts was observed in donors undergoing repeated aphereses, resulting in persistent and prolonged lymphopenia in 50\% of donors. No persistent monocytopenia over time and aphereses was observed. Kaplan-Meier estimate of the risk to develop persistent lymphopenia after multiple aphereses was 21\% +/- 6\% at 2 months, 38\% +/- 8\% at 4 months, and 64\% +/- 10\% at 12 months. After Cox regression analysis, previous PBSC harvest remained the unique factor associated with the risk for persistent lymphopenia.

Conclusions: Monitoring the potential long-term effects of repeated aphereses in hematopoietic stem cell donors appears important. Selecting young bone marrow donors for subsequent DLI significantly reduces the risk for acute and prolonged lymphopenias.},
	author = {Nicolini, FE and Wattel, E and Michallet, AS and Bourgeot, JP and Tremisi, JP and Hequet, O and Michallet, M}
}
