@article {308,
	title = {Detection of Mycobacterium tuberculosis in corneas from donors with active tuberculosis disease through polymerase chain reaction and culture},
	journal = {Br J Ophthalmol},
	volume = {94},
	year = {2010},
	note = {Catedral, E J Santos, R E Padilla, M D B Fajardo-Ang, C England The British journal of ophthalmology},
	month = {Jul},
	pages = {894 - 7},
	edition = {40110},
	abstract = {OBJECTIVE: To determine if Mycobacterium tuberculosis can be detected in corneas of donors who have active tuberculosis (TB) disease using polymerase chain reaction (PCR) or culture. DESIGN: This is a prospective cross-sectional study. PARTICIPANTS: 25 corneas (12 from TB-negative donors and 13 from TB-positive donors) from the Sta. Lucia International Eye Bank were submitted to the TB Research Laboratory, Medical Research Laboratory, Department of Medicine, UP-PGH, for diagnostic evaluation. INTERVENTION: Corneas were evaluated using PCR test for M tuberculosis. Acid-fast bacilli smear and culture were also done. MAIN OUTCOME MEASURES: Result of laboratory findings. RESULTS: Among 12 TB-negative donors, there were 2 (16.67\%) corneas that were PCR negative for M tuberculosis, whereas 10 (83.33\%) corneas were PCR positive for M tuberculosis. Among 13 TB-positive donors, there were 7 (53.85\%) corneas that were PCR negative for M tuberculosis, whereas 6 (46.15\%) corneas were PCR positive for M tuberculosis. All cultures were negative. CONCLUSION: PCR for M tuberculosis was positive for some TB-positive donors as well as TB-negative donors. However, all cultures were negative. It is recommended that further studies be done to investigate if recipients with PCR-positive corneas will eventually lead to disease transmission or not. It is further recommended that such findings be used to re-evaluate criteria for suitability of donors with tuberculosis.},
	keywords = {*Corneal Transplantation, *Tissue Donors, Adolescent, Adult, Aged, Aged, 80 and over, Child, Child, Preschool, Cornea / *microbiology, Cross-Sectional Studies, Female, Humans, Male, Middle Aged, Mycobacterium tuberculosis / *isolation \& purification, Polymerase Chain Reaction / methods, Prospective Studies, Tuberculosis, Pulmonary / *microbiology / transmission, Young Adult},
	issn = {1468-2079 (Electronic) 0007-1161 (Linking)},
	doi = {bjo.2008.153270 [pii] 10.1136/bjo.2008.153270},
	url = {internal-pdf://Catedral - detection of MTB-0998998528/Catedral - detection of MTB.pdf},
	author = {Catedral,E. J. and Santos,R. E. and Padilla,M. D. and Fajardo-Ang,C.}
}
