@article {482,
	title = {Estimating the relapse stage in chronic myeloid leukaemia patients after allogeneic stem cell transplantation by the amount of BCR-ABL fusion transcripts detected using a new real-time polymerase chain reaction method},
	journal = {Br J Haematol},
	volume = {113},
	year = {2001},
	note = {0007-1048 (Print) Journal Article},
	month = {Jun},
	pages = {1072 - 5},
	abstract = {We have used a new single-step real-time reverse transcription polymerase chain reaction (RT-PCR) method to quantify BCR-ABL transcripts, thereby estimating the relapse stage in chronic myeloid leukaemia patients after allogeneic transplants. In 402 samples from 172 patients, BCR-ABL expression was determined and normalized, using the GAPDH housekeeping gene product as an endogenous reference. In our real-time RT-PCR assay, serial dilutions of RNA of the K562 cell line remained positive down to 7.5 pg. The median normalized BCR-ABL amount differed significantly (P < 0.001) between the various disease stages and was 0.06\% (range 0.001-1.55\%), 3.2\% (range 1.4-5.6\%) and 21.5\% (range 6.8 -827\%) in 17 patients with a molecular relapse, in eight patients with a cytogenetic relapse and in 10 patients with a haematological relapse respectively.},
	keywords = {*Hematopoietic Stem Cell Transplantation, Adolescent, Adult, Comparative Study, Cytogenetic Analysis, Female, Fusion Proteins, bcr-abl / *genetics, Humans, In Situ Hybridization, Fluorescence, Leukemia, Myeloid, Chronic / *diagnosis / *surgery, Male, Middle Aged, Polymerase Chain Reaction / methods, Recurrence, Research Support, Non-U.S. Gov{\textquoteright}t, Transplantation, Homologous},
	author = {Elmaagacli,A. H. and Freist,A. and Hahn,M. and Opalka,B. and Seeber,S. and Schaefer,U. W. and Beelen,D. W.}
}
