@article {559,
	title = {Molecular monitoring of organ recipients from cancer-affected donors by detection of circulating tumor cells},
	journal = {Transplantation Proceedings},
	volume = {36},
	year = {2004},
	note = {Gabusi, E Corti, B D{\textquoteright}Errico, A Ridolfi, L Ercolani, G Venettoni, S Nanni Costa, A Grigioni, W F Transplant Proc. 2004 Jun;36(5):1344-7.},
	month = {Jun},
	pages = {1344 - 7},
	type = {Research Support, Non-U.S. Gov{\textquoteright}t},
	edition = {38183},
	abstract = {We have initiated regular molecular monitoring based on nested RT-PCR detection of circulating tumor cells for monitoring recipients of organs from cancer-affected donors in Italy (in the context of a "Donation Safety and Donated Organ Quality" project organized by the Centro Nazionale Trapianti). Five patients are being monitored. For two patients who each received a kidney from a single donor with prostate adenocarcinoma, RT-PCR was performed using PSA mRNA. For three recipients of organs (two livers and one kidney) from donors with renal cell carcinoma, RT-PCR was performed using cytokeratine 18 and 19 mRNA. Blood samples from five healthy subjects were used as negative controls. After a median monitoring time of 26 months (range 8 to 32), none of the regular 3-month assays has tested positive. This pilot study suggests that detection of circulating tumor cells by nested RT-PCR may provide a feasible molecular monitoring, which might assist decision making regarding other forms of clinical surveillance.},
	keywords = {*Kidney Neoplasms / pathology, *Prostatic Neoplasms / pathology, *Tissue Donors, Base Sequence, DNA Primers, Humans, Kidney Transplantation / *pathology, Liver Transplantation / *pathology, Male, Monitoring, Physiologic / *methods, Reverse Transcriptase Polymerase Chain Reaction, Sensitivity and Specificity},
	issn = {0041-1345 (Print) 0041-1345 (Linking)},
	doi = {10.1016/j.transproceed.2004.05.031},
	author = {Gabusi,E. and Corti,B. and D{\textquoteright}Errico,A. and Ridolfi,L. and Ercolani,G. and Venettoni,S. and Nanni Costa,A. and Grigioni,W. F.}
}
