@article {664,
	title = {Cornea as a tissue reservoir of Trypanosoma cruzi},
	journal = {Parasitol Res},
	volume = {100},
	year = {2007},
	note = {Herrera, Leidi Martinez, Clara Carrasco, Hernan Jansen, Ana Maria Urdaneta-Morales, Servio Research Support, Non-U.S. Gov{\textquoteright}t Germany Parasitology research Parasitol Res. 2007 May;100(6):1395-9. Epub 2006 Dec 20.},
	month = {May},
	pages = {1395 - 9},
	edition = {39072},
	abstract = {Trypanosoma cruzi causal agent of Chagas{\textquoteright} disease is a paninfective parasite of mammals transmitted through skin fecal contamination by Triatominae vectors. Studies of alternative routes for infection are scarce; therefore, eye infection should be important, because of the eye{\textquoteright}s high blood irrigation and brain proximity, as port of entry of the parasite. Trypanosoma cruzi parasites and/or their genetic material in ocular and adjacent muscle tissues were studied in batches of six NMRI mice (15 g) and Trichomys apereoides, an ancient caviomorph (250 g) inoculated with T. cruzi metacyclics from Brazilian (2) and Venezuelan (3) isolates genetically typified as T. cruzi I and II. Two animals/batch in the acute or chronic phase were killed and necropsies of cardiac and skeletal muscles, eyeball, and surrounding ocular muscle were processed for hematoxylin-eosine staining. Tissue parasitism was determined. DNA of the digested sections of the eyeball (5-10 mum) was extracted for T. cruzi k-DNA amplification by PCR, with S35 and S36 primers. The PCR products were analyzed. The average of maximum values of parasitemia of all infected animals was of 10(5) trypomastigotes/ml blood. Skeletal muscle and heart were colonized in patent infection for all isolates. Amastigote nests were found in corneal tissue of 2/3 of the used isolates and adjacent ocular muscle and connective tissue were parasitized. Trypanosoma cruzi k-DNA (330-bp band) was observed in ocular tissue of 4/6 of the isolates studied in both animal models. Investigations concerning infection of the eye globe tissues by T. cruzi are extremely scarce. The presence of stages of T. cruzi and/or its genetic products in ocular tissues indicate a broad colonization from a systemic infection. The results show the ocular environment as a possible appropriate microniche for T. cruzi and emphasize the risk of transmitting T. cruzi by ocular fluids and by parasitized cornea through transplants.},
	keywords = {Animals, Cornea / *parasitology, Disease Reservoirs / parasitology / *veterinary, Mice, Rodentia / parasitology, Trypanosoma cruzi / *isolation \& purification},
	issn = {0932-0113 (Print) 0932-0113 (Linking)},
	doi = {10.1007/s00436-006-0403-9},
	url = {internal-pdf://Herrera - Cornea as reservoir Chagas-4256415488/Herrera - Cornea as reservoir Chagas.pdf},
	author = {Herrera,L. and Martinez,C. and Carrasco,H. and Jansen,A. M. and Urdaneta-Morales,S.}
}
