@article {1359,
	title = {Safety of sperm washing and ART outcome in 741 HIV-1-serodiscordant couples},
	journal = {Hum Reprod},
	volume = {22},
	year = {2007},
	note = {Savasi, V Ferrazzi, E Lanzani, C Oneta, M Parrilla, B Persico, T England Human reproduction (Oxford, England) Hum Reprod. 2007 Mar;22(3):772-7. Epub 2006 Nov 15.},
	month = {Mar},
	pages = {772 - 7},
	edition = {39038},
	abstract = {BACKGROUND: To evaluate the safety of sperm washing and assisted reproduction technique (ART) outcome offered to serodiscordant couples with a human immunodeficiency virus-1 (HIV-1)-positive male. METHODS: Sperm washing was performed and checked by RT-PCR on each semen sample before its fresh usage. Intrauterine insemination (IUI) or IFV/ICSI was offered according to fertility profile of each couple. Non-infected women underwent HIV testing 2 weeks before each procedure and for up to 6 months after. RESULTS: Seven hundred and forty-one couples entered the study of a possible 2011 serodiscordant couples counselled over 4 years. Superovulation and IUI were performed in 581 couples, where the pregnancy rate per cycle and pregnancy rate per couple were 19 and 78\%, respectively, with multiple pregnancy rate being 4\%. One hundred and sixty couples were treated by IVF/ICSI, where pregnancy rate per cycle and per couple were 22 and 41\%, respectively, with multiple pregnancy rate being 10\%. All female partners were still HIV-1 negative at follow-up. CONCLUSION: Sperm washing within a programme of reproductive counselling was proved to be safe in this large series of serodiscordant couples. The overall pregnancy rate (70.3\%), independent of the procedure used (IUI or IVF/ICSI), justifies the effort of the medical team in setting up and implementing dedicated centres and of the individual patient in seeking a safe pregnancy.},
	keywords = {*HIV Seropositivity, *Reproductive Techniques, Assisted, Acquired Immunodeficiency Syndrome / *prevention \& control, Female, Fertilization in Vitro, Humans, Insemination, Artificial, Homologous, Male, Pregnancy, Pregnancy Rate, Specimen Handling / *methods, Sperm Injections, Intracytoplasmic, Spermatozoa / *virology, Tissue Donors, Treatment Outcome, Viral Load},
	issn = {0268-1161 (Print) 0268-1161 (Linking)},
	doi = {del422 [pii] 10.1093/humrep/del422},
	author = {Savasi,V. and Ferrazzi,E. and Lanzani,C. and Oneta,M. and Parrilla,B. and Persico,T.}
}
