02214nas a2200253   4500008003900000245014700039210006900186300000700255490000700262520144300269100001901712700001901731700001601750700002201766700001801788700001401806700001401820700001401834700001401848700001501862700001701877700001701894856004901911       2015                          d00aOrgan Transplantation Hepatitis C Investigation Team. Transmission of Hepatitis C Virus From Organ Donors Despite Nucleic Acid Test Screening.0 aOrgan Transplantation Hepatitis C Investigation Team Transmissio  a270 v153 aNucleic acid testing (NAT) for hepatitis C virus (HCV) is recommended for screening of organ donors, yet not all donor infections may be detected. We describe three US clusters of HCV transmission from donors at increased risk for HCV infection. Donor's and recipients' medical records were reviewed. Newly infected recipients were interviewed. Donor-derived HCV infection was considered when infection was newly detected after transplantation in recipients of organs from increased risk donors. Stored donor sera and tissue samples were tested for HCV RNA with high-sensitivity quantitative PCR. Posttransplant and pretransplant recipient sera were tested for HCV RNA. Quasispecies analysis of hypervariable region-1 was used to establish genetic relatedness of recipient HCV variants. Each donor had evidence of injection drug use preceding death. Of 12 recipients, 8 were HCV-infected-6 were newly diagnosed posttransplant. HCV RNA was retrospectively detected in stored samples from donor immunologic tissue collected at organ procurement. Phylogenetic analysis showed two clusters of closely related HCV variants from recipients. These investigations identified the first known HCV transmissions from increased risk organ donors with negative NAT screening, indicating very recent donor infection. Recipient informed consent and posttransplant screening for blood-borne pathogens are essential when considering increased risk donors.1 aSuryaprasad, A1 aBasavaraju, SV1 aHocevar, SN1 aTheodoropoulos, N1 aZuckerman, RA1 aHayden, T1 aForbi, JC1 aPegues, D1 aLevine, M1 aMartin, SI1 aKuehnert, MJ1 aBlumberg, EA  uhttps://www.notifylibrary.org/reference/5111