03027nas a2200373   4500008004500000022001400045245007200059210006900131260002300200300000700223490000700230520189500237653001002132653002002142653001102162653001802173653003102191653003602222653005402258653004902312653001102361653000702372653002502379653006102404653002602465100001702491700001402508700001502522700001502537700001502552700001902567700001802586856004902604       2008                        Engldsh    a1527-646500aChronic hepatitis E virus infection in liver transplant recipients.0 aChronic hepatitis E virus infection in liver transplant recipien  aUnited StatescApr  a470 v143 aHepatitis E virus (HEV) infection is known to run a self-limiting course. Sporadic cases of acute hepatitis due to infection with HEV genotype 3, present in pig populations, are increasingly recognized. Zoonotic transmission seems infrequent. The entity of unexplained chronic hepatitis after liver transplantation has been recognized. Detection of HEV in 2 liver transplant recipients triggered a review of these cases. Freeze-stored sera were available for retrospective analysis. HEV antibodies were determined. For virus detection and identification, a fragment of the gene encoding the major capsid protein (open reading frame 2) was amplified by reverse-transcription polymerase chain reaction and sequenced to identify the genotype. Two months after liver transplantation, case A developed unexplained chronic hepatitis, which developed into cirrhosis. Retransplantation followed 7 years later, after which chronic hepatitis recurred. In retrospect, HEV RNA was present in serum 3 weeks after the first transplantation and remained present afterwards. HEV RNA was also present in retransplant liver tissue. HEV antibodies appeared late after retransplantation. Case B developed unexplained chronic hepatitis 7 years after transplantation. Retransplantation was needed 5 years later, after which no signs of hepatitis recurred. In retrospect, the period of chronic hepatitis up to the retransplantation coincided with HEV RNA in serum. In case B, antibodies developed, the viral load was much lower than in case A, and the virus seemed to be cleared after retransplantation. Genotyping in both cases revealed 2 unique strains of genotype 3. In conclusion, chronic HEV infection may develop in immunosuppressed patients, who may then serve as long-term carriers of the virus. We hypothesize that HEV may be the cause of chronic hepatitis in liver transplant recipients. (c) 2008 AASLD.10aAdult10aChronic Disease10aFemale10aFreeze Drying10aHepatitis B / su [Surgery]10aHepatitis E / ep [Epidemiology]10aHepatitis E virus / ip [Isolation & Purification]10aHepatolenticular Degeneration / su [Surgery]10aHumans10aIM10aLiver Function Tests10aLiver Transplantation / sn [Statistics & Numerical Data]10aRetrospective Studies1 aHaagsma, E B1 aBerg, A P1 aPorte, R J1 aBenne, C A1 aVennema, H1 aReimerink, J H1 aKoopmans, M P  uhttps://www.notifylibrary.org/reference/1794