05972nas a2200181   4500008004500000022001400045245008800059210006900147260001300216300001200229490000700241520540900248653002305657100002105680700002505701700001505726856004905741       2013                        Engldsh    a0041-113200aBacillus cereus septicemia attributed to a matched unrelated bone marrow transplant0 aBacillus cereus septicemia attributed to a matched unrelated bon  cFebruary  a394-3970 v533 a<p>BACKGROUND: Hematopoietic cell transplantation (HCT) is performed in more than 25,000 patients annually. Clinically significant bacterial transmission from HCT products is rare. CASE REPORT: A 36-year-old male of Asian descent with chronic myelogenous leukemia developed sepsis leading to acute renal failure and disseminated intravascular coagulation during infusion of matched unrelated donor bone marrow. This product later tested positive for Bacillus cereus. DISCUSSION: This HCT product traveled 31 hours at room temperature before arriving at the transplant center. Reducing transport times, transporting at 4[degrees]C, and enhancing bacterial surveillance of HCT products may increase the ability to detect bacterial proliferation from transport. CONCLUSION: To prevent a similar case in the future, we will begin Gram staining all HCT products in transit more than 24 hours to alert physicians of the need for prophylactic antibiotic therapy. Copyright (C) 2013 Blackwell Publishing Ltd.; References: 1. National Marrow Donor Program. 2011. [cited 2012 May 14]. Available from: URL: http://www.marrow.org. 2. Kamble R, Pant S, Selby GB, Kharfan-Dabaja MA, Sethi S, Kratochvil K, Kohrt N, Ozer H. Microbial contamination of hematopoietic progenitor cell grafts-incidence, clinical outcome, and cost-effectiveness: an analysis of 735 grafts. Transfusion 2005;45:874-8. 3. Schwella N, Zimmermann R, Heuft HG, Blasczyk R, Beyer J, Rick O, Kreissig C, Zingsem J, Eckstein R, Siegert W. Microbiologic contamination of peripheral blood stem cell autografts. Vox Sang 1994;67:32-5. 4. Foundation for the Accreditation of Cellular Therapy. FACT-JACIE international standards for cellular therapy product collection, processing and administration. 4th). ed. Omaha, NE: Foundation for the Accreditation of Cellular Therapy; 2011. 5. Patterson HC, Sturtevant O, Schott D, Alyea EP 3rd, Onderdonk AB, Kao GS. Isolation of non-typhoidal Salmonella from a haematopoietic progenitor cell product. Transfus Med 2010;20:424-5. 6. Webb IJ, Coral FS, Andersen JW, Elias AD, Finberg RW, Nadler LM, Ritz J, Anderson KC. Sources and sequelae of bacterial contamination of hematopoietic stem cell components: implications for the safety of hematotherapy and graft engineering. Transfusion 1996;36:782-8. 7. Reich-Slotky R, Wu F, Della-Latta P, Savage DG, Schwartz J. Application of pulsed-field gel electrophoresis to identify the source of bacterial contamination of peripheral blood progenitor cell products. Transfusion 2008;48:2409-13. 8. Helgason E, Okstad OA, Caugant DA, Johansen HA, Fouet A, Mock M, Hegna I, Kolst[phi] AB. Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis-one species on the basis of genetic evidence. Appl Environ Microbiol 2000;66:2627-30. 9. Bottone EJ. Bacillus cereus, a volatile human pathogen. Clin Microbiol Rev 2010;23:382-98. 10. Kotiranta A, Lounatmaa K, Haapasalo M. Epidemiology and pathogenesis of Bacillus cereus infections. Microbes Infect 2000;2:189-98. 11. Kobayashi K, Kami M, Ikeda M, Kishi Y, Murashige N, Tanosaki R, Mori S, Takaue Y. Fulminant septicemia caused by Bacillus cereus following reduced-intensity umbilical cord blood transplantation. Haematologica 2005;90:ECR06. 12. Cunha G, Leao L, Pimenta F. Bacterial contamination of random-donor platelets in a university hospital in the midwestern region of Brazil. Transfusion 2008;48:282-5. 13. Sasahara T, Hayashi S, Morisawa Y, Sakihama T, Yoshimura A, Hirai Y. Bacillus cereus bacteremia outbreak due to contaminated hospital linens. Eur J Clin Microbiol Infect Dis 2011;30:219-26. 14. Dohmae S, Okubo T, Higuchi W, Takano T, Isobe H, Baranovich T, Kobayashi S, Uchiyama M, Tanabe Y, Itoh M, Yamamoto T. Bacillus cereus nosocomial infection from reused towels in Japan. J Hosp Infect 2008;69:361-7. 15. Kao GS, Kim HT, Daley H, Ritz J, Burger SR, Kelley L, Vierra-Green C, Flesch S, Spellman S, Miller J, Confer D. Validation of short-term handling and storage conditions for marrow and peripheral blood stem cell products. Transfusion 2011;51:137-47. 16. Walther-Wenke G. Incidence of bacterial transmission and transfusion reactions by blood components. Clin Chem Lab Med 2008;46:919-25. 17. Holme S, McAlister MB, Ortolano GA, Chong C, Cortus MA, Jacobs MR, Yomtovian R, Freundlich LF, Wenz B. Enhancement of a culture-based bacterial detection system (eBDS) for platelet products based on measurement of oxygen consumption. Transfusion 2005;45:984-93. 18. Rock G, Neurath D, Toye B, Sutton D, Giulivi A, Bormanis J, Olberg B, Holme S, Wenz B, Ortolano G, Nelson E. The use of a bacteria detection system to evaluate bacterial contamination in PLT concentrates. Transfusion 2004;44:337-42. 19. Ortolano GA, Freundlich LF, Holme S, Russell RL, Cortus MA, Wilkins K, Nomura H, Chong C, Carmen R, Capetandes A, Wenz B. Detection of bacteria in WBC-reduced PLT concentrates using percent oxygen as a marker for bacteria growth. Transfusion 2003;43:1276-85. 20. Yazer MH, Stapor D, Triulzi DJ. Use of the RQI test for bacterial screening of whole blood platelets. Am J Clin Pathol 2010;133:564-8. 21. Sogaard M, Norgaard M, Schonheyder HC. First notification of positive blood cultures and the high accuracy of the gram stain report. J Clin Microbiol 2007;45:1113-7. 22. National Marrow Donor Program. Standards. 20th ed. 2009. [cited 2012 May 14]. Available from: URL: http://www.marrow.org.</p>10aClinical Medicine.1 aKelley, James, M1 aOnderdonk, Andrew, B1 aKao, Grace  uhttps://www.notifylibrary.org/reference/1805