03089nas a2200325   4500008004100000020001400041024001700055245014200072210006900214260031200283300001600595490000700611520178300618100002402401700001902425700002202444700002302466700002102489700001702510700002102527700001902548700002402567700002002591700002202611700002202633700002102655700002002676700001802696856004902714       2008                        eng d  a0041-1132  awdn, 041736000aA case of transfusion-transmitted hepatitis E caused by blood from a donor infected with hepatitis E virus via zoonotic food-borne route.0 acase of transfusiontransmitted hepatitis E caused by blood from   aCopyright (C) 2008 Blackwell Publishing Ltd.bFrom the Hokkaido Red Cross Blood Center, Sapporo; the Center for Gastroenterology, Teine Keijinkai Hospital, Sapporo; the Department of Medical Sciences, Toshiba General Hospital, Tokyo; the Third Department of Internal Medicine, Asahikawa Medical Collegc2008  a1368 - 13750 v483 aBACKGROUND: Five cases of transfusion transmission of hepatitis E virus (HEV) have been reported so far. The infection routes of the causative donors remain unclear, however. Also, the progress of virus markers in the entire course of HEV infection has not been well documented., STUDY DESIGN AND METHODS: Nucleic acid testing was performed by real-time reverse transcription-polymerase chain reaction targeting the open reading frame 2 region of HEV. Full-length nucleotide sequences of HEV RNA were detected by direct sequencing., RESULTS: Lookback study of a HEV-positive donor revealed that the platelets (PLTs) donated from him 2 weeks previously contained HEV RNA and were transfused to a patient. Thirteen relatives including the donor were ascertained to enjoy grilled pork meats together in a barbecue restaurant 23 days before the donation. Thereafter, his father died of fulminant hepatitis E and the other 6 members showed serum markers of HEV infection. In the recipient, HEV was detected in serum on Day 22 and reached the peak of 7.2 log copies per mL on Day 44 followed by the steep increase of alanine aminotransferase. Immunoglobulin G anti-HEV emerged on Day 67; subsequently, hepatitis was resolved. HEV RNA sequences from the donor and recipient were an identical, Japan-indigenous strain of genotype 4. HEV RNA was detectable up to Day 97 in serum, Day 85 in feces, and Day 71 in saliva., CONCLUSION: A transfusion-transmitted hepatitis E case by blood from a donor infected via the zoonotic food-borne route and the progress of HEV markers in the entire course are demonstrated. Further studies are needed to clarify the epidemiology and the transfusion-related risks for HEV even in industrialized countries., Copyright (C) 2008 Blackwell Publishing Ltd.1 aMatsubayashi, Keiji1 aKang, Jong-Hon1 aSakata, Hidekatsu1 aTakahashi, Kazuaki1 aShindo, Motohiro1 aKato, Masaru1 aSato, Shinichiro1 aKato, Toshiaki1 aNishimori, Hiroyuki1 aTsuji, Kunihiko1 aMaguchi, Hiroyuki1 aYoshida, Jun-ichi1 aMaekubo, Hiroshi1 aMishiro, Shunji1 aIkeda, Hisami  uhttps://www.notifylibrary.org/reference/4070