02471nas a2200253   4500008004100000020001400041024001700055245006400072210006300136260029900199300001600498490000700514520146700521100002401988700002202012700001602034700002202050700001702072700001802089700002202107700001902129700002002148856004902168       2003                        eng d  a0041-1132  awdn, 041736000aWest Nile virus infection transmitted by blood transfusion.0 aWest Nile virus infection transmitted by blood transfusion  a(C) 2003 Blackwell Science Ltd.bEpidemic Intelligence Service Branch, Division of Applied Public Health Training, Epidemiology Program Office, Centers for Disease Control and Prevention, Atlanta, Georgia; the Division of Healthcare Quality Promotion, the Division of Vector-Borne Infectic2003  a1018 - 10220 v433 aBACKGROUND: A patient with transfusion-transmitted West Nile virus (WNV) infection confirmed by viral culture of a blood component is described. A 24-year-old female with severe postpartum hemorrhage developed fever, chills, headache, and generalized malaise after transfusion of 18 units of blood components; a serum sample and the cerebrospinal fluid tested positive for the presence of WNV IgM antibodies. An investigation was initiated to determine a possible association between transfusion and WNV infection., STUDY DESIGN AND METHODS: Blood donors were assessed for recent infection through questionnaires and WNV testing of serum samples. Whole-blood retention segments and untransfused blood components were sent to the CDC to test for the presence of WNV through PCR (TaqMan, Applied Biosystems), IgM ELISA, plaque reduction neutralization testing, and viral culture., RESULTS: Three of 15 available donor retention segments were WNV PCR-positive. WNV was recovered from one associated blood component. The implicated donor was symptomatic near the time of donation; serology confirmed WNV IgM seroconversion., CONCLUSION: Seroconversion of a symptomatic donor, the presence of viral genetic material in an associated whole-blood retention segment, and recovery of WNV from an associated component provides compelling evidence for transfusion-acquired infection. This report has important implications for blood safety., (C) 2003 Blackwell Science Ltd.1 aHarrington, Theresa1 aKuehnert, Matthew1 aKamel, Hany1 aLanciotti, Robert1 aHand, Sheryl1 aCurrier, Mary1 aChamberland, Mary1 aPetersen, Lyle1 aMarfin, Anthony  uhttps://www.notifylibrary.org/reference/4152