02298nas a2200217   4500008004100000020001400041024001700055245016400072210006900236260020000305300001400505490000700519520139300526100001501919700002401934700001801958700001301976700002201989700002002011856004902031       2014                        eng d  a0041-1132  awdn, 041736000aClostridium perfringens in apheresis platelets: an unusual contaminant underscores the importance of clinical vigilance for septic transfusion reactions (CME).0 aClostridium perfringens in apheresis platelets an unusual contam  a(C) 2014 John Wiley & Sons, Ltdb(1) National Headquarters, Biomedical Services,(2) Holland Laboratory, American Red Cross, Rockville, Maryland,(3) American Red Cross, Louisville, Kentucky,c2014  a857 - 8620 v543 aBACKGROUND: Posttransfusion sepsis is typically caused by aerobic bacteria in apheresis platelets (PLTs) that escape detection by routine quality control cultures performed on every donation before components are distributed. We report the first case to implicate an anaerobic isolate, Clostridium perfringens, in apheresis PLTs and investigate its detection in vitro by approved tests., STUDY DESIGN and METHODS: The C. perfringens strain was inoculated at high (10-100 colony-forming units [CFUs]/mL) or low (1-10 CFUs/mL) concentrations into apheresis PLTs and evaluated for growth over 5 to 7 days by qualitative plate cultures, culture-based assays (BacT/ALERT 3D), and rapid (PLT PGD) tests., RESULTS: C. perfringens grew in only 3 of 8 apheresis PLT units after inoculation at either high (2 units) or low (1 unit) concentrations. The PGD test detected the isolate after 5 days in 1 unit with 4.7 x 105 CFUs/mL but failed at five other time points in units with greater than 105 CFUs/mL., CONCLUSION: C. perfringens demonstrated variable growth in spiked PLTs and was not consistently detected by a rapid test even when high levels of contamination were present. The case underscores the importance of direct observation during transfusion, appropriate clinical management, and immediate reporting of suspected septic reactions to the blood center., (C) 2014 John Wiley & Sons, Ltd1 aEder, Anne1 aMeena-Leist, Claire1 aHapip, Cheryl1 aDy, Beth1 aBenjamin, Richard1 aWagner, Stephen  uhttps://www.notifylibrary.org/reference/4160