02806nas a2200313   4500008004100000020001400041024001700055245016700072210006900239260018200308300001200490490000800502520166700510653001402177653002502191653002202216653001902238653004102257100001702298700001902315700001502334700001502349700002202364700001202386700001602398700001402414700001502428856004902443       2015                        eng d  a0042-9007  axli, 041360600aAnalysis of leucocyte antibodies, cytokines, lysophospholipids and cell microparticles in blood components implicated in post-transfusion reactions with dyspnoea.0 aAnalysis of leucocyte antibodies cytokines lysophospholipids and  a(C) 2015 John Wiley & Sons, Ltdb(1)Department of Immunohematology and Immunology of Transfusion Medicine, Institute of Hematology and Transfusion Medicine, Warsaw, Polandc2015  a27 - 360 v1083 aBackground and Objectives: Post-transfusion reactions with dyspnoea (PTR) are major causes of morbidity and death after blood transfusion. Transfusion-related acute lung injury (TRALI) and transfusion-associated circulatory overload (TACO) are most dangerous, while transfusion-associated dyspnoea (TAD) is a milder respiratory distress. We investigated blood components for immune and non-immune factors implicated in PTR., Material and Methods: We analysed 464 blood components (RBCs, PLTs, L-PLTs, FFP) transfused to 271 patients with PTR. Blood components were evaluated for 1/antileucocyte antibodies, 2/cytokines: IL-1[beta], IL-6, IL-8, TNF-[alpha], sCD40L, 3/lysophosphatidylcholines (LysoPCs), 4/microparticles (MPs) shed from plateletes (PMPs), erythrocytes (EMPs) and leucocytes (LMPs)., Results: Anti-HLA class I/II antibodies or granulocyte-reactive anti-HLA antibodies were detected in 18[middle dot]2% of blood components (RBC and FFP) transfused to TRALI and in 0[middle dot]5% of FFP transfused to TAD cases. Cytokines and LysoPCs concentrations in blood components transfused to PTR patients did not exceed those in blood components transfused to patients with no PTR. Only EMPs percentage in RBCs transfused to patients with TRALI was significantly higher (P < 0[middle dot]05) than in RBCs transfused to patients with no PTR., Conclusion: Immune character of PTR was confirmed mainly in 1/5 TRALI cases. Among non-immune factors, only MPs released from stored RBCs are suggested as potential mediators of TRALI. Our results require further observations in a more numerous and better defined group of patients., (C) 2015 John Wiley & Sons, Ltd10aCytokines10aleucocyte antibodies10alysophospholipids10amicroparticles10apulmonary post-transfusion reactions1 aMaslanka, K.1 aUhrynowska, M.1 aLopacz, P.1 aWrobel, A.1 aSmolenska-Sym, G.1 aGuz, K.1 aLachert, E.1 aOstas, A.1 aBrojer, E.  uhttps://www.notifylibrary.org/reference/4351