TY - JOUR T1 - Detection of Mycobacterium tuberculosis in corneas from donors with active tuberculosis disease through polymerase chain reaction and culture JF - Br J Ophthalmol Y1 - 2010 A1 - Catedral,E. J. A1 - Santos,R. E. A1 - Padilla,M. D. A1 - Fajardo-Ang,C. KW - *Corneal Transplantation KW - *Tissue Donors KW - Adolescent KW - Adult KW - Aged KW - Aged, 80 and over KW - Child KW - Child, Preschool KW - Cornea / *microbiology KW - Cross-Sectional Studies KW - Female KW - Humans KW - Male KW - Middle Aged KW - Mycobacterium tuberculosis / *isolation & purification KW - Polymerase Chain Reaction / methods KW - Prospective Studies KW - Tuberculosis, Pulmonary / *microbiology / transmission KW - Young Adult AB - OBJECTIVE: To determine if Mycobacterium tuberculosis can be detected in corneas of donors who have active tuberculosis (TB) disease using polymerase chain reaction (PCR) or culture. DESIGN: This is a prospective cross-sectional study. PARTICIPANTS: 25 corneas (12 from TB-negative donors and 13 from TB-positive donors) from the Sta. Lucia International Eye Bank were submitted to the TB Research Laboratory, Medical Research Laboratory, Department of Medicine, UP-PGH, for diagnostic evaluation. INTERVENTION: Corneas were evaluated using PCR test for M tuberculosis. Acid-fast bacilli smear and culture were also done. MAIN OUTCOME MEASURES: Result of laboratory findings. RESULTS: Among 12 TB-negative donors, there were 2 (16.67%) corneas that were PCR negative for M tuberculosis, whereas 10 (83.33%) corneas were PCR positive for M tuberculosis. Among 13 TB-positive donors, there were 7 (53.85%) corneas that were PCR negative for M tuberculosis, whereas 6 (46.15%) corneas were PCR positive for M tuberculosis. All cultures were negative. CONCLUSION: PCR for M tuberculosis was positive for some TB-positive donors as well as TB-negative donors. However, all cultures were negative. It is recommended that further studies be done to investigate if recipients with PCR-positive corneas will eventually lead to disease transmission or not. It is further recommended that such findings be used to re-evaluate criteria for suitability of donors with tuberculosis. VL - 94 ER - internal-pdf://Catedral - detection of MTB-0998998528/Catedral - detection of MTB.pdf CP - 7 N1 - Catedral, E J Santos, R E Padilla, M D B Fajardo-Ang, C England The British journal of ophthalmology ID - 308 ER -