Risk of contamination of germplasm during cryopreservation and cryobanking in IVF units.

TitleRisk of contamination of germplasm during cryopreservation and cryobanking in IVF units.
Publication TypeJournal Article
Year of Publication2009
AuthorsBielanski A, Vajta G
JournalHum Reprod
Pagination2457 - 67
Date PublishedOct
Accession Number19561041
KeywordsBiological Specimen Banks, Cryopreservation, Cryoprotective Agents, Embryo Culture Techniques, Embryo, Mammalian, Equipment Contamination, Female, Fertilization in Vitro, Humans, Male, Nitrogen, Oocytes, Risk Assessment, Semen, Specimen Handling, Spermatozoa

Cryopreservation of sperm, embryos and, more recently, oocytes plays an important and increasing role in assisted reproduction, due to improvements of old, and introduction of new technologies. In parallel, concerns are increasing about the technical and biological safety of these procedures. However, published data regarding the confirmed or theoretical hazards of these procedures are sparse and sometimes contradictory. The purpose of this review will summarize data and opinions about one of the most disputed risks, the potential hazard of contamination and disease transmission through cryopreservation. Special attention is concentrated on the weak points of the technology including open vitrification systems, sterilization of liquid nitrogen and safety of commonly used storage tanks including straws and cryovials. Suggestions are also made for practical measures to avoid these dangers while preserving the benefits and perspectives of new cryopreservation technologies.

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