Safety of sperm washing and ART outcome in 741 HIV-1-serodiscordant couples

TitleSafety of sperm washing and ART outcome in 741 HIV-1-serodiscordant couples
Publication TypeJournal Article
Year of Publication2007
AuthorsSavasi V, Ferrazzi E, Lanzani C, Oneta M, Parrilla B, Persico T
JournalHum Reprod
Volume22
Issue3
Pagination772 - 7
Date PublishedMar
ISSN0268-1161 (Print) 0268-1161 (Linking)
Accession Number17107974
Keywords*HIV Seropositivity, *Reproductive Techniques, Assisted, Acquired Immunodeficiency Syndrome / *prevention & control, Female, Fertilization in Vitro, Humans, Insemination, Artificial, Homologous, Male, Pregnancy, Pregnancy Rate, Specimen Handling / *methods, Sperm Injections, Intracytoplasmic, Spermatozoa / *virology, Tissue Donors, Treatment Outcome, Viral Load
Abstract

BACKGROUND: To evaluate the safety of sperm washing and assisted reproduction technique (ART) outcome offered to serodiscordant couples with a human immunodeficiency virus-1 (HIV-1)-positive male. METHODS: Sperm washing was performed and checked by RT-PCR on each semen sample before its fresh usage. Intrauterine insemination (IUI) or IFV/ICSI was offered according to fertility profile of each couple. Non-infected women underwent HIV testing 2 weeks before each procedure and for up to 6 months after. RESULTS: Seven hundred and forty-one couples entered the study of a possible 2011 serodiscordant couples counselled over 4 years. Superovulation and IUI were performed in 581 couples, where the pregnancy rate per cycle and pregnancy rate per couple were 19 and 78%, respectively, with multiple pregnancy rate being 4%. One hundred and sixty couples were treated by IVF/ICSI, where pregnancy rate per cycle and per couple were 22 and 41%, respectively, with multiple pregnancy rate being 10%. All female partners were still HIV-1 negative at follow-up. CONCLUSION: Sperm washing within a programme of reproductive counselling was proved to be safe in this large series of serodiscordant couples. The overall pregnancy rate (70.3%), independent of the procedure used (IUI or IVF/ICSI), justifies the effort of the medical team in setting up and implementing dedicated centres and of the individual patient in seeking a safe pregnancy.

DOI10.1093/humrep/del422
Notify Library Reference ID1359

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